Production of microbial keratinase using a newly isolated strain of Stenotrophomonas maltophilia and its characterization and applications.

Biodegradation of keratin containing wastes viz. bird feathers, nails, hairs, animalwool is a low-cost, nutrient-rich biotechnological process that turns this plentiful waste into substance. It sustainable green approach towards the solution environmental threats created by these wastes. This study aimed to screen for potential keratinase-producing microorganisms and optimize physicochemical parameters produce keratinase its characterization. Samples were collected from various poultry farms Surat (India). Screening keratinase-production was carried using feather basal medium. Among samples, 16 isolates showed keratinolytic activity in primary screening skim milk agar medium among which, nine Isolate KA1a gave highest yield identified as strain Stenotrophomonas maltophilia (NCBI Accession number MT478451), based on 16S rRNA sequencing. delivered enzyme production (108 U/ml) with feathers (2.2%), NaCl (0.5%), K2HPO4(0.3%), KH2PO4(0.4%), pH7, 10% inoculum young cell mass, at 37°C 24 h under shaking conditions. Optimum temperature 40°C pH 7.0. Enzyme stability over different up 90 min. applications detergent additive, animal feed, organic fertilizer.